Anti-STIM1 (N-terminal) antibody produced in rabbit
SIGMA/S6072 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: STIM1 Antibody - Anti-STIM1 (N-terminal) antibody produced in rabbit; Stim1 Antibody; Anti-GOK; Anti-Stromal interaction molecule I
Product Type: Chemical
Western Blotting JOURNAL CITATION: Identification of Stim1 as a candidate gene for exaggerated sympathetic response to stress in the stroke-prone spontaneously hypertensive rat. By: Ferdaus, M. Z., Xiao, B., et al. in PLoS One, 2014. PubMed ID: 24736434 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0095091), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 5 μg/mL Rabbit Anti-STIM1 (N-terminal) (Cat. No. S6072). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
Enhanced Validation-By Independent Antibodies Immunofluorescence. HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 5 μg/mL Anti-STIM1 (N-terminal) antibody produced in Rabbit (Cat. No. S6072) (B). The antibody developed using Goat Anti-Rabbit IgG, Cy3 Conjugate antibody, and 5 μg/mL Anti-STIM1 (C-terminal) antibody produced in Rabbit (Cat. No. S6197) (A). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 Conjugate antibody. Results Two Anti-STIM1 antibodies, S6197 (A) and S6072 (B), target different regions of STIM1 show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunoblotting Whole extract of 3T3 cells was separated on SDS-PAGE and probed with Rabbit Anti-STIM1 (N terminal) (Cat. No. S6072). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 0.5 μg/mL antibody 3. 0.5 μg/mL antibody + STIM 1 (N terminal) immunizing peptide (human, 61-74)
Immunoblotting Whole extract of K562 cells was separated on SDS-PAGE and probed with 1 μg/mL Rabbit Anti-STIM1 (N terminal) (Cat. No. S6072). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Immunoblotting Whole extract of PC12 cells was separated on SDS-PAGE and probed with 1 μg/mL Rabbit Anti-STIM1 (N terminal) (Cat. No. S6072). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 90 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2.5-5 μg using extracts of rat PC12 cells. |
| indirect immunofluorescence: 5-10 μg/mL using human HeLa cells. | |
| western blot: 2-4 μg/mL using whole extract of mouse 3T3 cells. | |
| UniProt accession no. | Q13586  |
| Application: | Anti-STIM1 (N-terminal) antibody produced in rabbit has been used in: • western blotting • immunoprecipitation • immunofluorescence |
| Biochem/physiol Actions: | STIM1 (stromal interaction molecule 1) is required for the activation of store-operated Ca2+ influx. Overexpression of STIM1 and Orai1 markedly increases the calcium release-activated calcium (CRAC) current (I-CRAC). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | STIM1 (stromal interaction molecule 1) is a conserved single-pass transmembrane protein, which is expressed ubiquitously in a wide variety of human primary and transformed cell types. STIM1 is modified by phosphorylation and N-linked glycosylation. STIM1 localized predominantly in the membrane of the endoplasmic reticulum (ER). It contains an N-terminal EF hand motif located in the ER lumen and appears to function as a sensor of ER Ca2+ levels. |
| Immunogen: | synthetic peptide corresponding to amino acids 61-74 of human STIM1, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, 7.4 pH, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |