Sephadex® G-100
SIGMA/S6147 - DNA grade, BioReagent, Molecular Biology, medium
CAS Number: 9050-94-6
MDL Number: MFCD00002744
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: S6147-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: S6147-25G
| application(s) | life science and biopharma |
| bead diameter | 40-120 μm |
| compatibility | Cytiva |
| foreign activity | DNase, RNase, none detected |
| form | beads |
| grade | Molecular Biology |
| matrix active group | phase |
| pH | 2-10 |
| pore size | exclusion limit (25 bp dsDNA; (rA)27) |
| product line | BioReagent |
| Quality Level | 200  |
| storage temp. | room temp |
| swelling | 1 g swells to 9-11 mL |
| technique(s) | buffer exchange: suitable |
| Application: | Sephadex® G-100 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify the enzyme polyphenoloxidases isolated from banana by gel filteration. |
| Features and Benefits: | • Excellent recovery • Minimal sample dilution • Quick and easy to use |
| General description: | Sephadex® G-100, Medium is an often-cited gel filtration medium for desalting and buffer exchange of very large molecules. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required. |
| Legal Information: | Sephadex is a registered trademark of Cytiva |
| Packaging: | 25, 100 g in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 23151817 |