Anti-STIM1 (C-terminal) antibody produced in rabbit
SIGMA/S6197 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-GOK; Anti-Stromal interaction molecule 1
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 5 μg/mL Rabbit Anti-STIM1 (C-terminal) (Cat. No. S6197). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
Enhanced Validation-By Independent Antibodies Immunofluorescence. HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 5 μg/mL Anti-STIM1 (N-terminal) antibody produced in Rabbit (Cat. No. S6072) (B). The antibody developed using Goat Anti-Rabbit IgG, Cy3 Conjugate antibody, and 5 μg/mL Anti-STIM1 (C-terminal) antibody produced in Rabbit (Cat. No. S6197) (A). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 Conjugate antibody. Results Two Anti-STIM1 antibodies, S6197 (A) and S6072 (B), target different regions of STIM1 show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunoblotting Whole extract of 3T3 cells was separated on SDS-PAGE and probed with Rabbit Anti-STIM1 (C-terminal) (Cat. No. S6197). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 1 μg/mL antibody + STIM 1 (C-terminal) immunizing peptide (human, 657-683) 3. 2 μg/mL antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 90 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2.5-5 μg using extracts of rat PC12 cells. |
| indirect immunofluorescence: 2.5-5 μg/mL using human HeLa cells. | |
| western blot: 2-4 μg/mL using whole extract of mouse 3T3 cells. | |
| UniProt accession no. | Q13586  |
| Application: | Anti-STIM1 (C-terminal) antibody produced in rabbit has been used in: • western blot • immunoprecipitation • immunofluorescence • immunohistochemistry |
| Biochem/physiol Actions: | Stromal interaction molecule 1 (STIM1) is required for the activation of store-operated Ca2+ influx. STIM1 undergoes rapid oligomerization and redistributes into discrete spots (punctae) that move towards and accumulate in the cell periphery, possibly to activate Orai1 that is located in the plasma membrane. Overexpression of STIM1 and Orai1 together markedly increases the calcium release-activated channels (CRAC) current (I-CRAC). Dynamic assembly of TRPC1-STIM1-Orai1 ternary complex plays a major role in the activation of store-operated calcium (SOC) channel in response to internal Ca2+ store depletion. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Stromal interaction molecule 1 (STIM1) is a conserved single-pass transmembrane protein. STIM1 is expressed ubiquitously in a wide variety of human primary and transformed cell types. STIM1 localizes predominantly in the membrane of the endoplasmic reticulum (ER). It contains an N-terminal EF hand motif located in the ER lumen and appears to function as a sensor of ER Ca2+ levels. |
| Immunogen: | synthetic peptide corresponding to amino acids 657-683 of human STIM1, conjugated to KLH via an N-terminal residue. The corresponding sequence is identical in rat, mouse, bovine, monkey, and dog. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |