Streptavidin−Cy3™ from Streptomyces avidinii
SIGMA/S6402 - buffered aqueous solution
Synonym: Streptavidin Cy3
MDL Number: MFCD00164141
Product Type: Chemical
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| conjugate | CY3 conjugate |
| extent of labeling | 3-9 mol fluorochrome per mol protein |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 |
| Application: | Streptavidin−Cy3™ from Streptomyces avidinii has been used: • in double immunofluorescence staining to detect biotinylated lectin • for insulin and C-peptide staining in immunofluorescence analysis • in double immunofluorescence to verify the presence of cells filled with biocytin • in conjunction with biotinylated antibodies in immunohistochemistry and immunocytochemistry • with the traditional rhodamine filter arrangements due to the spectral overlap |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Spectral Characteristics of Cy3 Dye:• Absorbance Max 552 nm • Emission Max 565 nm • Molar Extinction Coefficient 1.3 x 10-5 M-1cm-1 |
| General description: | Streptavidin is a homotetramer protein. One molecule of streptavidin binds four molecules of biotin by a non-covalent interaction that is essentially irreversible. Streptavidin has a high affinity for biotin and is extensively used as a labeling tool and for studies involving binding proteins. |
| Legal Information: | Cy3 is a trademark of Cytiva |
| Packaging: | 1 mL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |