SSC Buffer 20× Concentrate
SIGMA/S6639 - for Northern and Southern blotting, solution
Synonym: 20X SSC Buffer; SSC hybridization buffer; Saline-sodium citrate buffer
Product Type: Chemical
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| application(s) | diagnostic assay manufacturing |
| color | colorless |
| description | Saline-Sodium Citrate buffer, made with ultrapure water |
| foreign activity | DNAse, none detected |
| Endonuclease, none detected | |
| Exonuclease, none detected | |
| NICKase, none detected | |
| RNAse, none detected | |
| form | solution |
| grade | Molecular Biology |
| pH | 6.9 —7.1 |
| Quality Level | 200  |
| sterility | 0.2 μm filtered |
| storage temp. | room temp |
| Application: | SSC Buffer 20× Concentrate has been used • as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence • as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA and northern blotting of microRNA • as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon |
| Features and Benefits: | • Convenient ready-to-use liquid • Multi-purpose use in hybridizations |
| General description: | SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization. |
| Preparation Note: | SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 41105319 |