Monoclonal Anti-S100A2 antibody produced in mouse
SIGMA/S6797 - clone SH-L1, ascites fluid
Synonym: Anti-S100L
MDL Number: MFCD00677383
Product Type: Chemical
Immunofluorescence Madin-Darby bovine kidney (MDBK) cells were fixed and permeabilized with 0.2% Triton™ X-100 followed by 0.2% Formaldehyde and by acetone. Fixed cells were stained with 1:2,000 Monoclonal Anti-S100A2 antibody produced in Mouse, Clone: SH-L1 (Cat. No. S6797) followed by 1:40 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
Western Blotting Whole extract of MDBK cells were separated on SDS-PAGE and probed with Monoclonal Anti-S100A2 antibody produced in Mouse, Clone: SH-L1 (Cat. No. S6797). The antibody was developed using 1:1,000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A1293). Lanes 1. 1:1,000 antibody 2. 1:2,000 antibody 3. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | SH-L1, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | lizard, goat, rat, bovine, human, feline, frog, canine, pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunohistochemistry (frozen sections): suitable | |
| immunoprecipitation (IP): suitable using native preparations | |
| indirect immunofluorescence: 1:1,000 using cultured Madin-Darby bovine kidney (MDBK) cells | |
| western blot: 1:1,000 | |
| UniProt accession no. | P29034  |
| Application: | Monoclonal Anti-S100A2 antibody has been used in enzyme linked immunosorbent assay (ELISA), immunoblotting, immunoprecipitation, immunohistology, western blotting. |
| Biochem/physiol Actions: | S-100 binds to calcium and zinc ions reversibly at physiologic pH and ionic strength, followed by a conformational change in the molecule. S-100 is a cell-growth regulator, increasing the membrane permeability to cations under physiologic conditions, stimulation of nucleolar RNA polymerase activity, interaction with the tumor suppressor protein p53 and as a carrier of proteins and free fatty acids in adipocytes. High levels of S100A2 are found also in the MDBK (Madin-Darby bovine kidney) cell line and in human glioma. |
| Biochem/physiol Actions: | The antibody recognizes an epitope located on S100A2 (S100L is the old terminology) in a Ca2+ ion-dependent manner. The product does not react with other members of the EF-hand family such as calmodulin, parvalbumin, intestinal calcium-binding protein, S100A6 (calcyclin), caltropin, the α chain of S-100 (i.e. in S-100a and S-100ao), or the β chain (i.e. in S-100a and S-100b). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-S100A2 (S100L) (mouse IgG1 isotype) is derived from the SH-L1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. S-1002 is a set of small, thermolabile, highly acidic 10-20 kDa dimer proteins that are widely distributed in different tissues. The S-100 family consists of at least 10 members with a cell-type-specific expression pattern. It is a calcium-modulated protein. S100A2 is also called S100L. S100A2 has been shown to possess 43-47% homology with S-100α, S-100β and Calcyclin. It is expressed at high levels in kidney and lung, intermediate levels in muscle and low levels in brain and intestine. |
| Immunogen: | Ca2+-binding proteins from pig stomach tissue |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |