50 bp DNA Step Ladder
SIGMA/S7025 - for DNA electrophoresis
Synonym: DNA Ladder; DNA Ladder; Step Ladder, 50 bp, 17 Fragments, 50-3000 bp; Step Ladder, 50 bp, 17 Fragments, 50-3000 bp
Product Type: Chemical
S7025 on a 2% agarose gel
| form | liquid |
| Quality Level | 100  |
| storage temp. | −20°C |
| suitability | suitable for electrophoresis (DNA) |
| usage | 100 uses |
| Application: | Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels. |
| General description: | Sigma’s 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. For NA electrophoresis, 10 μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for Northern and Southern blotting. |
| Other Notes: | Contains blunt-ended fragments of DNA, 50-500 bp by 50, 600-900 bp by 100, and 1000-3000 bp by 1000 (17 bands). |
| Other Notes: | For optimal resolution, the recommended agarose gel concentration is 2.0%. |
| Other Notes: | Sigma’s 50 bp DNA Step Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 10 mM NaCl, and 1 mM EDTA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41105335 |