Superoxide Dismutase from bovine erythrocytes
SIGMA/S7446 - BioUltra, lyophilized powder, ≥4,500 units/mg protein, ≥97% (SDS-PAGE)
Synonym: SOD; Superoxide: superoxide oxidoreductase
CAS Number: 9054-89-1
EC Number: 232-943-0
MDL Number: MFCD00132404
Product Type: Chemical
SOD from bovine erythrocytes is a homodimeric non-covalently bound protein with two 16.3 kDa subunits of 151 amino acids. Each monomer has one intrachain disulfide and one free sulfhydryl, two copper atoms and two zinc atoms.
Multiple enzymatic scavengers are utilized by the cell to limit damage from reactive oxygen species. These scavengers include members of the superoxide dismutase (SOD) family, catalase, and glutathione peroxidase.
SOD catalyzes the conversion of superoxide radicals into hydrogen peroxide and molecular oxygen.
SDS-PAGE (4-20%) of SOD (Cat. No. S7446) under reducing conditions and stained with Coomassie blue.
| assay | ≥97% (SDS-PAGE) |
| composition | Protein, ≥95% biuret |
| form | lyophilized powder |
| mol wt | 32.5 kDa |
| product line | BioUltra |
| Quality Level | 200  |
| relevant disease(s) | arthritis |
| specific activity | ≥4,500 units/mg protein |
| storage temp. | −20°C |
| Application: | Superoxide Dismutase from bovine erythrocytes has been used in a study to assess activity parameters in adjuvant-induced arthritis. Superoxide Dismutase from bovine erythrocytes has also been used in a study to simplify the process of purifying superoxide dismutases using charge-controlled hydrophobic chromatography on 10-carboxydecyl Sepharose. It has also been used as a standard in SOD assay in mice liver andkidney samples. |
| Biochem/physiol Actions: | Superoxide Dismutase from bovine erythrocytes catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It serves as an antioxidant and plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice. |
| General description: | Research area: Cell signaling. Superoxide Dismutase (SOD) is found in allaerobic cells of the animals, plants, and microorganisms. It is a low molecularweight protein that exists as three different forms such as manganese SOD,copper–zinc SOD, and extracellular SOD. |
| Other Notes: | One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min. |
| Packaging: | 15000, 75000 units in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (SDS-PAGE) |
| activity | specific activity: ≥4,500 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.15.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |