Sphingomyelinase from Bacillus cereus
SIGMA/S7651 - lyophilized powder, ≥100 units/mg protein
Synonym: Sphingomyelin choline phosphohydrolase; Sphingomyelin phosphodiesterase
CAS Number: 9031-54-3
EC Number: 232-869-9
MDL Number: MFCD00132362
Product Type: Chemical
Stoichiometry: Sphingomyelin + H2O → N-Acylsphingosine + Choline phosphate
| composition | Protein, ~10% Lowry |
| form | lyophilized powder |
| mol wt | 36873 Da |
| Quality Level | 200  |
| specific activity | ≥100 units/mg protein |
| storage temp. | 2-8°C |
| Application: | Sphingomyelinase from Bacillus cereus has been used: • in the measurement of membrane capacitance in brain slices • for testing and confirming its silkworm-killing activity • in enzyme activity assay and for elucidating its effect on hydrolysis of sphingomyelin in complex lipid mixtures |
| Application: | Sphingomyelinase has been used in a study to investigate skeletal manifestations in pediatric and adult patients with Niemann Pick disease. |
| Biochem/physiol Actions: | Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin. |
| General description: | Sphingomyelinase from Bacillus cereus (Bc-Smase) is a Mg2+-containing metalloenzyme. Bacillus cereus sphingomyelinase induces hemolysis of sheep erythrocytes. |
| General description: | Sphingomyelinase modulates adenosine triphosphate (ATP)-independent endocytosis of fibroblast and large vesicles of macrophages. Sphingomyelinase causes hemolysis of human erythrocytes. It is also a potent virulence factor leading to invasive diseases. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C. |
| Packaging: | 10, 50 units in serum bottle |
| Physical form: | Lyophilized powder containing potassium phosphate buffer salts and stabilizer. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥100 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.4.12 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |