Anti-SMC6 antibody produced in rabbit
SIGMA/S7822 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-SMC6L1; Anti-Structural maintenance of chromosome 6; Anti-Structural maintenance of chromosomes 6-like 1
Product Type: Chemical
Western Blotting HEK-293T cells overexpressing OS9 extract were separated on SDS-PAGE and probed with Anti-SMC6 antibody produced in Rabbit (Cat. No. S7822). The antibody was developed using 1:30,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:500 antibody 2. 1:1,000 antibody 3. 1:500 antibody + inhibition with immunizing peptide 4. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 126 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat (predicted), mouse (predicted) |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:250-1:500 using whole cell extract of HEK293-T expressing SMC6 |
| UniProt accession no. | Q96SB8  |
| Application: | Anti-SMC6 antibody produced in rabbit may be used in immunoblotting. |
| Biochem/physiol Actions: | Anti-SMC6 (also known as SMC6L1) specifically recognizes SMC6. |
| Biochem/physiol Actions: | Structural maintenance of chromosomes protein 6 (SCM6) along with SMC5 is mainly involved in the cellular response to DNA damage in yeast as well as humans. The yeast SMC5/6 complex contains several non-SMC elements (NSE), including methyl methanesulfonate-sensitiv |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Structural maintenance of chromosomes protein 6 (SCM6) belongs to the structural maintenance of chromosomes family. There are at least six SMC family members that form three heterodimers in specific combinations. SCM6 gene is mapped to human chromosome 2p24.2. Structurally, SCM6 has DNA-binding domains at the N and C termini, a long coiled-coil domain, and a hinge region. |
| Immunogen: | synthetic peptide corresponding to amino acids 766-779 of human SMC6, conjugated to KLH via an N-terminal cysteine residue. This sequence differs by one amino acid in mouse, and by two amino acids in rat. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |