Sarcosine Oxidase from Bacillus sp.
SIGMA/S7897 - lyophilized powder, 25-50 units/mg solid
Synonym: Sarcosine: oxygen oxidoreductase (demethylating)
CAS Number: 9029-22-5
EC Number: 232-850-5
MDL Number: MFCD00132221
Product Type: Chemical
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| form | lyophilized powder |
| mol wt | 44 kDa |
| Quality Level | 200  |
| specific activity | 25-50 units/mg solid |
| storage temp. | −20°C |
| Application: | Sarcosine oxidase from Bacillus sp. has been used as a positive control for detection of proteins with covalently bound flavin adenine dinucleotide (FAD) by UV. It has also been used in co-immobilization with other enzymes on platinum electrode for preparation of creatinine biosensor. |
| Application: | Sarcosine oxidase has been used in a study as part of a multienzyme cascade, that when immobilized constructed amperometric biosensors. Sarcosine oxidase has also been used in a study to investigate oxidation of amines by flavoproteins. |
| General description: | Monomeric sarcosine oxidase (MSOX) is a flavoenzyme that catalyzes the oxidative demethylation of sarcosine (N-methylglycine) to yield glycine, formaldehyde, and hydrogen peroxide. Monomeric sarcosine oxidase can oxidize other secondary amino acids such as N-methyl-L-alanine, N-ethylglycine, and L-proline. |
| General description: | Sarcosine oxidase is a 44 kDa protein with covalently bound 1 mol of flavin adenine dinucleotide (FAD). It belongs to the family of amino acid oxidases with bound FAD. |
| Other Notes: | One unit will form 1.0 μmole of formaldehyde from sarcosine per min at pH 8.3 at 37 °C. |
| Packaging: | 1000 units in poly bottle |
| Physical form: | No stabilizers added |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 25-50 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.5.3.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |