Stem Cell Factor human
SIGMA/S7901 - SCF, recombinant, expressed in E. coli, powder, suitable for cell culture
Synonym: SCF; c-Kit ligand
MDL Number: MFCD00165757
Product Type: Chemical
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| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | powder |
| impurities | ≤1 EU/μgtested (LAL test) |
| mol wt | monomer 18.6 kDa ((165 aa)) |
| packaging | pkg of 10 μg |
| pkg of 50 μg | |
| potency | ≤15 ng/mL ED50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P21583  |
| Analysis Note: | The proliferative activity is tested in culture using TF1 human erythroleukemic cells. |
| Application: | Stem Cell Factor human has been used in • In vitro differentiation of equine embryonic stem cells • Chemotaxis detection system • Ex vivo expansion of umbilical cord blood CD34+ cells • Cytokine therapy |
| Biochem/physiol Actions: | Epidermal stem cell factor (SCF) is considered as an essential mast cell growth factor and a multifunctional cytokine. SCF mediates tumor development and progression. Upregulation of the SCF gene is observed in hepatocellular carcinoma. |
| Biochem/physiol Actions: | Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. |
| Biochem/physiol Actions: | Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |