Monoclonal Anti-Siah2 antibody produced in mouse
SIGMA/S7945 - clone Siah2-369, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-Seven in Absentia Homolog
MDL Number: MFCD08705601
Product Type: Chemical
A. Immunoblot Mouse brain extract was separated on SDS-PAGE and probed with Monoclonal Anti-SIAH2 (Cat No. S7945, 1 μg/mL). The antibody was developed with Rabbit Anti-Mouse Fab, Biotin conjugate and ExtrAvidin, Alkaline Phosphatase conjugate (Cat. No. E2636). B. Immunocytochemistry HEK-293T cells were labeled with Monoclonal Anti-SIAH2 (Cat. No. S7945, 20 μg/mL) followed by Goat Anti-Mouse Fab-FITC conjugate (Cat. No. F5262).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | Siah2-369, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~37 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | monkey, hamster, bovine, mouse, human, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 2-4 μg/mL using total cell extract of HeLa cells | |
| UniProt accession no. | O43255  |
| Application: | Monoclonal Anti-Siah2 antibody produced in mouse can be used in enzyme linked immunoassay (ELISA) and immunocytochemistry. It has also been used in immunohistochemistry and immunoblotting. |
| Biochem/physiol Actions: | Seven in absentia homologue (Siah) plays a role in the regulation of key proteins in the immune system such as TNF receptor associated factors (TRAF), vav guanine nucleotide exchange factor 1 (Vav1), and oct-binding factor 1 (OBF-1). Siah2 is important for the regulation of the PHD enzymes (prolylhydroxylases) that are responsible for the prolyhydroxylation of HIFα protein under hypoxia conditions. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Siah2 (mouse IgG1 isotype) is derived from the hybridoma Siah2-369 produced by the fusion of mouse myeloma cells (NS1 cell) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids 2-17 of human Siah2, conjugated to KLH. Seven in absentia homologue (Siah) is encoded by the gene mapped to human chromosome 3q25.1. Three different Siah genes exist in mice, Siah1a, Siah1b, and Siah2, while humans have only two genes, Siah1and Siah2. |
| Immunogen: | synthetic peptide corresponding to amino acids 2-17 of human Siah2, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |