Anti-SUMO-1 antibody produced in rabbit
SIGMA/S8070 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-GMP1; Anti-PIC1; Anti-SMT3C; Anti-SMT3H3; Anti-Sentrin-1; Anti-Small Ubiquitin-related Modifiier-1; Anti-UBL1
MDL Number: MFCD02096449
Product Type: Chemical
Immunofluorescence
HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 8 μg/mL Rabbit Anti-SUMO-1 (Cat. No. S8070). The antibody was developed using Goat Anti-Rabbit IgG, Atto 488 conjugate (Cat. No. 18772). Cells were counterstained with phalloidin rhodamine conjugate to stain Actin.
HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 8 μg/mL Rabbit Anti-SUMO-1 (Cat. No. S8070). The antibody was developed using Goat Anti-Rabbit IgG, Atto 488 conjugate (Cat. No. 18772). Cells were counterstained with phalloidin rhodamine conjugate to stain Actin.
Western Blotting HeLa cells nuclear extract were separated on SDS-PAGE and probed with Anti-SUMO-1 antibody produced in Rabbit (Cat. No. S8070). The antibody was developed using 1:10,000 Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545). Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. 1 μg/mL antibody + inhibition with immunizing peptide 4. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 14 kDa (unconjugated SUMO-1) |
| antigen 41 kDa (SUMO-1-GST) | |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 10-20 μg using a nuclear extract of HeLa cells |
| indirect immunofluorescence: 4-8 μg/mL using human HeLa cells | |
| microarray: suitable | |
| western blot: 0.5-1 μg/mL using nuclear extract of HeLa cells | |
| UniProt accession no. | P63165  |
| Application: | Anti-SUMO-1 antibody produced in rabbit has been used in immunoblotting assay. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | SUMO (small ubiquitin-like modifier) proteins are associated with a number of cellular processes. SUMOylation mediated by SUMO1 is involved in nuclear transport mechanism, apoptosis, regulation of cyclin-dependent kinase 6 and protein stability. SUMO1 regulates tumor suppressor p53 protein, a transcription factor that is termed as the “guardian of the genome” plays a key role in the cell cycle events. Unlike ubiquitination which directs proteins for degradation, SUMOylation aims to alter the stability, localization and the function of the target protein. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The SUMO-1 (small ubiquitin-like modifier 1) gene is mapped to human chromosome 2q33.1 and is highly conserved. The protein structure includes an α-helix core surrounded by four-stranded β-sheets. |
| Immunogen: | synthetic peptide corresponding to amino acid 1-16 located at the N-terminus of human SUMO-1, conjugated to KLH. The sequence is identical in many species including mouse, rat, dog, bovine, and highly conserved (single amino acid substitution) in chicken and Xenopus SUMO-1. It is not found in human SUMO-2 and SUMO-3. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |