SSPE Buffer

SIGMA/S8140 - for Northern and Southern blotting, powder blend

Synonym: Saline-Sodium Phosphate-EDTA Buffer

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-S8140-100ML	100 mL	$35.80 1/EA		Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

foreign activity	DNAse, none detected
	Endonuclease, none detected
	Exonuclease, none detected
	NICKase, none detected
	RNAse, none detected
form	powder blend
grade	Molecular Biology
pH	7.2-7.6 (20 × in water, high purity, ambient)
Quality Level	200
solubility	water, high purity: 20 ×, clear, colorless
storage temp.	room temp

Application:	Influence of Saline Buffers over the Stability of High-Annealed Gold Nanoparticles Formed on Coverslips for Biological and Chemosensing Applications.: This study explores the role of saline buffers in maintaining the stability of high-annealed gold nanoparticles used in biological and chemosensing applications. The findings highlight the potential for enhanced sensor performance in detecting biological molecules (Zhou & Ionescu, 2020 ). Quantification of Surface Etching by Common Buffers and Implications on the Accuracy of Label-Free Biological Assays.: This research assesses the impact of various buffers, including SSPE buffer, on the surface etching of substrates used in label-free biological assays. The results indicate that buffer-induced surface changes can significantly affect assay accuracy (Ahn et al., 2012 ).
Features and Benefits:	• Easy to reconstitute powder • Multi-purpose use in hybridizations
General description:	SSPE Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. This powder should be reconstituted to the indicated volume with molecular biology grade water before use. The resulting 20x liquid can be used straight or diluted as needed.
Packaging:	1 L in poly bottle
Packaging:	100 mL in poly bottle
Preparation Note:	Final SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	room temp
UNSPSC	41105319

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