Anti-Substance P Receptor antibody produced in rabbit
SIGMA/S8305 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-NK1R; Anti-SPR
MDL Number: MFCD01634202
Product Type: Chemical
Immunohistochemistry-immunofluorescence JOURNAL CITATION: CO2-evoked release of PGE2 modulates sighs and inspiration as demonstrated in brainstem organotypic culture. By: Forsberg, D., Horn, Z., et al. in Elife, 2016. PubMed ID: 27377173 Image collected and cropped by CiteAb from the following publication, (https://elifesciences.org/articles/14170), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: CO2-evoked release of PGE2 modulates sighs and inspiration as demonstrated in brainstem organotypic culture. By: Forsberg, D., Horn, Z., et al. in Elife, 2016. PubMed ID: 27377173 Image collected and cropped by CiteAb from the following publication, (https://elifesciences.org/articles/14170), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry JOURNAL CITATION: CO2-evoked release of PGE2 modulates sighs and inspiration as demonstrated in brainstem organotypic culture. By: Forsberg, D., Horn, Z., et al. in Elife, 2016. PubMed ID: 27377173 Image collected and cropped by CiteAb from the following publication, (https://elifesciences.org/articles/14170), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Rat striatum, lateral septum, tuberculum olfactorium tissue stained with Rabbit Anti-Substance P Receptor (Cat. No. S8305). From Dr. Shai Shoham, Research Laboratory, Sarah Herzog Memorial Hospital, Jerusalem, Israel
Immunohistochemistry Rat striatum, lateral septum, tuberculum olfactorium tissue stained with Rabbit Anti-Substance P Receptor (Cat. No. S8305). From Dr. Shai Shoham, Research Laboratory, Sarah Herzog Memorial Hospital, Jerusalem, Israel
Immunohistochemistry Rat striatum, lateral septum, tuberculum olfactorium tissue stained with Rabbit Anti-Substance P Receptor (Cat. No. S8305). From Dr. Shai Shoham, Research Laboratory, Sarah Herzog Memorial Hospital, Jerusalem, Israel
Immunohistochemistry Rat hippocampal dentate gyrus tissue stained with Rabbit Anti-Substance P Receptor (Cat. No. S8305). From Dr. Shai Shoham, Research Laboratory, Sarah Herzog Memorial Hospital, Jerusalem, Israel
Western Blotting Whole extract of Rat brain were separated on SDS-PAGE and probed with Anti-Substance P Receptor antibody produced in Rabbit (Cat. No. S8305). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:4,000 antibody 2. 1:2,000 antibody 3. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 46 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse, guinea pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (frozen sections): 1:5,000 using 4% paraformaldehyde (with picric acid/glutaraldehyde)-fixe |
| microarray: suitable | |
| western blot (chemiluminescent): 1:2,000 using a rat brain membrane fraction extract | |
| UniProt accession no. | P25103  |
| Application: | Anti-substance P receptor antibody produced in rabbit has been used in immunohistochemistry. |
| Biochem/physiol Actions: | Substance P (SP) exhibits pro-inflammatory, endocrine, neuromodulatory and trophic activities. Substance P receptor (SPR) upon binding to its ligand SP, facilitates extravasation of granulocytes and in inflammation and tissue derangement. In addition, activation of this receptor also enhances metabolism of phosphoinositol, leading to synthesis of inositol-1,4,5-trisphosph |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Substance P receptor (SPR), also known as neurokinin receptor 1 (NK-1 R), is a member of the G-protein-coupled class of seven transmembrane domain receptors. SPR gene with five exons, spanning 45-60kb on genomic DNA, is mapped to human chromosome 2. SPR is highly expressed in central nervous system. |
| Immunogen: | synthetic peptide corresponding to the C-terminal of NK1R of rat origin (amino acids 393-407). This sequence is highly conserved in mouse, guinea pig, and human NK1R, but diverges in other tachykinin receptor subtypes NK2R and NK3R. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |