Anti-Sirt2 antibody produced in rabbit
SIGMA/S8447 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-SIR2L; Anti-SIR2L2; Anti-Sirtuin (silent mating type information regulation 2 homolog); Anti-Sirtuin 2
MDL Number: MFCD07784768
Product Type: Chemical
Immunoprecipitation Rabbit Anti-Sirt2 (Cat. No. S8447) was used to immunoprecipitate Sirt2 from COS7 cells overexpressing human Sirt2. Detection antibody: Rabbit Anti-Flag (Cat. No. F7425) followed by Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 2 μg antibody 2. Negative control: 2 μg non-relevant antibody 3. Positive control: whole extract of COS7 cells overexpressing human Sirt2
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 μg/mL Rabbit Anti-Sirt2 (Cat. No. S8447). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
Immunoblotting Whole extracts of mouse brain (lanes 1, 3) and rat brain (lanes 2, 4) were separated on SDS-PAGE and probed with Rabbit Anti-Sirt2 (Cat. No. S8447). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 2 μg/mL 2. 2 μg/mL 3. 2 μg/mL + Sirt2 immunizing peptide (mouse, 341-352) 4. 2 μg/mL + Sirt2 immunizing peptide (mouse, 341-352)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 37:43 kDa (rat) |
| antigen 43 kDa (human) | |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-5 μg using extract of COS7 cells or 293 cells expressing recombinant human SIRT2 protein. |
| indirect immunofluorescence: 5-10 μg/mL using human HeLa cells. | |
| western blot: 1-2 μg/mL using whole extracts of mouse and rat brain. | |
| UniProt accession no. | Q8IXJ6  |
| Application: | Anti-Sirt2 antibody has been used in western blotting and immunofluorescence. |
| Application: | Rabbit polyclonal anti-Sirt2 antibody may be used in several applications including immunoblotting, immunoprecipitation, and immunofluorescence. |
| Biochem/physiol Actions: | Sirtuin 2 (SIRT2) maintains the integrity of the genome. Inhibition of SIRT2 can lead to neuroprotection in cellular and invertebrate models of Huntington′s disease. SIRT2 can deacetylate lys40 of α-tubulin both in vitro and in vivo. Knockdown of SIRT2 by small interfering RNA (siRNA) leads to tubulin hyperacetylation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Rabbit polyclonal anti-Sirt2 antibody recognizes mouse, rat (37/43 kDa), and human (43 kDa) Sirt2. Detection of the Sirt2 bands by immunoblotting is specifically inhibited with the immunizing peptide. |
| General description: | Sirt2 is an NAD+-dependent tubulin deacetylase. Two Sirt2 isoforms exist as the result of alternative splicing. Sirt2 is overexpressed during mitosis and is multiply phosphorylated at the G2/M transition of the cell cycle, indicating that it plays a role in the control of mitotic exit in the cell cycle. Although Sirt2 resides predominantly in the cytoplasm, it becomes enriched in the nucleus during the cell cycle where it is associated with mitotic structures. Sirt2 was found to be an oligodendroglial protein that affects cell differentiation through deacetylating α-tubulin. |
| General description: | Sirtuin 2 (SIRT2) is a cytoplasmic protein, which is part of the sirtuin family of proteins. SIRT2 colocalizes with microtubules. This gene is located on human chromosome 19q13.2. |
| Immunogen: | synthetic peptide corresponding to amino acids 341-352 of mouse Sirt2, conjugated to KLH. The corresponding sequence is identical in rat and differs by one amino acid in human Sirt2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |