Sphingomyelinase from Staphylococcus aureus
SIGMA/S8633 - buffered aqueous glycerol solution, 100-300 units/mg protein (Lowry)
Synonym: Sphingomyelin choline phosphohydrolase; Sphingomyelin phosphodiesterase
CAS Number: 9031-54-3
MDL Number: MFCD00132362
Product Type: Chemical
Stoichiometry: Sphingomyelin + H2O → N-Acylsphingosine + Choline phosphate
| form | buffered aqueous glycerol solution |
| Quality Level | 200  |
| specific activity | 100-300 units/mg protein (Lowry) |
| storage temp. | 2-8°C |
| Application: | Sphingomyelinase from Staphylococcus aureus has been used to: • induce neurotoxicity in rat cortical cultures to study the protective effects of minocycline • determine the concentration of sphingomyelin from serum samples • enhance sphingomyelinase activity to study PARK9-mediated exosome biogenesis |
| Biochem/physiol Actions: | Bacterial sphingomyelinase is active at neutral pH. When used in cell culture in vitro, it hydrolyzes the sphingomyelin on the outer leaflet of the plasma membrane and produces ceramide that is lipid-soluble. Sphingomyelinase is the key enzyme in the sphingomyelinase/ceramide pathway, which is implicated in the pathogenesis of several neurodegenerative disorders. |
| Biochem/physiol Actions: | Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin. |
| Other Notes: | One unit will hydrolyze 1.0 μmol of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C. |
| Packaging: | 25, 50 units in glass bottle |
| Physical form: | Solution in 50% glycerol containing 0.25 M phosphate buffer, pH 7.5 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 100-300 units/mg protein (Lowry) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.4.12 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |