Sodium bicarbonate solution
SIGMA/S8761 - solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture
Synonym: aqueous sodium bicarbonate
CAS Number: 144-55-8
MDL Number: MFCD00003528
Product Type: Chemical
| form | solution (7.5%) |
| impurities | endotoxin, tested |
| InChI | 1S/CH2O3.Na/c2-1(3)4;/h(H |
| InChI key | UIIMBOGNXHQVGW-UHFFFAOYSA |
| pH | 7.8-8.2 |
| product line | BioReagent |
| Quality Level | 400  |
| SMILES string | [Na+].OC([O-])=O |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Sodium bicarbonate solution has been used:• as a component of Hanks′ buffered salt solution (HBSS) and recording medium for culturing of tissue explants from rats or mice • as a component of Dulbecco′s modified Eagle′s medium (DMEM) to culture mice tissue explants • as a component of Minimum Essential Media (MEM) to study the cytotoxicity against human colorectal adenocarcinoma cells and mammary gland adenocarcinoma • to prepare collagen mix droplets for 3D collagen sprouting assay • to prepare collagen matrix for rat epidermal keratinocyte (REK) organotypic culture |
| General description: | Sodium bicarbonate (NaHCo3) is a commonly used alkaline buffering agent(5) in laboratories. The addition of this buffer to the cell culture medium aids in maintaining the appropriate pH by using the carbon dioxide present in the incubator. In addition to its buffering properties, Sodium bicarbonate also acts as a source of sodium and aids in sodium gluconate synthesis during the fermentation process. |
| Preparation Note: | Prepared in cell culture grade water. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12161702 |