Monoclonal Anti-Sodium Channel, Pan antibody produced in mouse
SIGMA/S8809 - ~1 mg/mL, clone K58/35, purified immunoglobulin
Synonym: Sodium Channel Antibody
MDL Number: MFCD02097413
Product Type: Chemical
Immunohistochemistry-immunofluorescence JOURNAL CITATION: A Brain-Derived Neurotrophic Factor-Based p75NTR Peptide Mimetic Ameliorates Experimental Autoimmune Neuritis Induced Axonal Pathology and Demyelination. By: Gonsalvez, D. G., Tran, G., et al. in Eneuro, 2017. PubMed ID: 28680965 Image collected and cropped by CiteAb from the following publication, (http://eneuro.sfn.org/lookup/doi/10.1523/ENEURO.0142-17.2017), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | K58/35, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | mammals |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (frozen sections): suitable |
| indirect immunofluorescence: 1 μg/mL using sciatic and optic nerve sections; cultured hypocampal neurons | |
| western blot: 1 μg/mL using crude brain membrane prep (rat) | |
| UniProt accession no. | P35498  |
| Application: | Monoclonal Anti-Sodium Channel, Pan antibody produced in mouse has been used in: • immunohistochemistry(1: • immunostaining (1:200) • immunofluorescence • immunocytochemistry (1:100) |
| Biochem/physiol Actions: | The sequence recognized by this clone is identical in all known vertebrate Na+ channels. In cultured hippocampal neurons, the antibody specifically stains the axon initial segment. In sciatic and optic nerves, it stains the nodes of Ranvier. |
| Biochem/physiol Actions: | Voltage-gated sodium channels/VGSCs (Nav) participates in nociceptive transmission. They play a key role in regulating the exchange between the extracellular and intracellular spaces. VGSCs are crucial for the initiation and firing of action potentials. They participate in neuronal excitability. Hence mutations in the VGSC genes results in several epileptic disorders. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Voltage-gated sodium channels/VGSCs (Nav) are transmembrane proteins that are initiated by the depolarization of the membrane. They are heteromultimers with large pore-forming α-subunits and smaller β-subunits. |
| Immunogen: | synthetic peptide CTEEQKKYYNAMKKLGSKK from the intracellular III-IV loop of Na+ channels |
| Legal Information: | Sold under exclusive license from SUNY Stony Brook. |
| Physical form: | Solution in 10 mM Tris, pH 7.5, containing 0.1 m NaCl, 30% glycerol, and 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |