SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free
SIGMA/S8830 - for use in purification of Histidine-tagged proteins
Synonym: Protease Inhibitor Cocktail
MDL Number: MFCD00677817
Product Type: Chemical
SigmaFAST Protease Inhibitor Cocktail Tablet, EDTA-free: Cat. No. S8830
SigmaFAST Protease Inhibitor Cocktail Tablet, EDTA-free. Protease inhibitor cocktail tablets are also available in other formulations.
| biological source | bovine lung |
| form | tablet |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Application: | The broad specificity of SIGMAFAST™ Protease Inhibitor Cocktail Tablets makes them suitable for use in the purification of histidine-tagged proteins and as a general-purpose protease inhibitor cocktail. The tablets can also be used as components of buffers for cell lysis, and have been optimized and tested for use with HIS-Select® media. |
| Biochem/physiol Actions: | Inhibits serine, cysteine, aspartic, and metalloproteases. |
| Features and Benefits: | EDTA-Free: Contains no strong metal chelators, such as EDTA, which might interfere with IMAC processing. Pepstatin A: Contains a unique, proprietary formulation that includes a pepstatin A salt for additional protection not normally obtained in a tablet format. Broad Specificity: Inhibits serine, cysteine, aspartic, and metalloproteases, providing broad protection for protein samples. High Yield: One tablet makes 100 mL of cocktail, and is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract. Long Stability: The tablets are stable as supplied for at least 2 years at 2–8 °C, and the reconstituted protease inhibitor solution (1´ or 10´) is stable for at least 2 weeks at 2–8 °C. |
| General description: | SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free is a water-soluble tablet formulation designed to inhibit serine, cysteine, aspartic and metalloproteases. The tablets contain no strong metal chelators, such as EDTA, and are compatible with sample processing by immobilized metal affinity chromatography (IMAC). The tablets also contain a unique, proprietary formulation that includes a pepstatin A salt for additional protection. |
| Legal Information: | HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | AEBSF Bestatin E-64 Pepstatin A Phosphoramidon Leupeptin Aprotinin |
| Physical form: | Tablet that is water soluble |
| Preparation Note: | One tablet makes 100 mL of cocktail. One tablet is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract. |
| Preparation Note: | The tablets are stable as supplied for at least 2 years at 2–8 °C. The reconstituted protease inhibitor solution (1´ or 10´) is stable for at least 2 weeks at 2–8 °C. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 |
| Precautionary statements | P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313 |
| Hazard Codes | C |
| Risk Statements | 34 |
| Safety Statements | 26-36/37/39-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |