Safranin O
SIGMA/S8884 - certified by the BSC
Synonym: Basic Red 2; Cotton Red; Gossypimine; Safranin T; Safranin Y or A
CAS Number: 477-73-6
Empirical Formula (Hill Notation): C20H19ClN4
Molecular Weight: 350.84
EC Number: 207-518-8
MDL Number: MFCD00011759
Linear Formula: C20H19ClN4
Product Type: Chemical
Safranin O
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: S8884-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: S8884-25G
| agency | certified by the BSC |
| application(s) | diagnostic assay manufacturing hematology histology |
| color | green to very dark green |
| composition | Dye content, ≥80% |
| form | powder |
| InChI | 1S/C20H18N4.ClH/c1-12-8-1 |
| InChI key | OARRHUQTFTUEOS-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | [Cl-].Cc1cc2nc3cc(C)c(N)c |
| solubility | H2O: 1 mg/mL |
| storage temp. | room temp |
| technique(s) | microbe id | staining: suitable |
| Application: | Certified as a stain for chromosomes in the Flemming triple stain; to stain gram negative bacteria in the Gram stain procedure; Safranine-fast green FCF stain for plant cells. |
| Application: | Safranin O has been used in the staining of extraocular muscle mesenchymal stem cells (EOM-MSC) for the detection of cartilage, mucin and mast cell granules It has also been used as a mediator dye in cytochrome P450 spectral assay. |
| Application: | Safranin O has been used to stain matrix proteoglycans to study cartilaginous tissue structural integrity in mice. It has also been used to stain chondrogenically differentiated human bone marrow stromal cells. |
| General description: | Safranin O is a metachromatic, cationic dye. It is used as a counterstain in Gram staining. The stain colors Gram-negative bacteria pink to red and has no effect on Gram-positive bacteria. It is also used to assess glycosaminoglycans (GAGs) in cartilage. The dye imparts an intense red to GAGs. It easily binds to polyanions. |
| Packaging: | 25, 100 g in glass bottle |
| Symbol |  GHS05 |
| Signal word | Danger |
| Hazard statements | H318 |
| Precautionary statements | P280 - P305 + P351 + P338 + P310 |
| Hazard Codes | Xi |
| Risk Statements | 41 |
| Safety Statements | 26-39 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | room temp |
| Colour Index Number | 50240 |
| UNSPSC | 12171500 |