Sulforhodamine B sodium salt

SIGMA/S9012 - Technical grade

Synonym: Acid red 52; Acid Rhodamine B

CAS Number: 3520-42-1
Empirical Formula (Hill Notation): C₂₇H₂₉N₂NaO₇S₂
Molecular Weight: 580.65
EC Number: 222-529-8
MDL Number: MFCD00010180
Linear Formula: C₂₇H₂₉N₂NaO₇S₂
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-S9012-5G	5 g	$140.00 1/EA	Add To Favorites
45-S9012-25G	25 g	$483.00 1/EA	Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

ε_max	≥85000 at 554-560 nm
application(s)	diagnostic assay manufacturing hematology histology
composition	Dye content, ~75%
form	powder
InChI	1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1
InChI key	SXQCTESRRZBPHJ-UHFFFAOYSA-M
Quality Level	200
SMILES string	[Na+].CCN(CC)c1ccc2c(OC3=CC(C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](CC)CC)c1
storage temp.	room temp

Application:	Sulforhodamine B (SRB) is known for use in SRB assay for the determination of in vitro cytotoxicity through staining and the observation of live cells, proliferation and drug screening. It has been used in the SRB assay to evaluate the effect of mouthwashes on protein content in primary cultures of human fibroblasts. It is also suitable as a microscopic tracer for the assessment of blood-brain barrier integrity by post mortem inspection of frozen sections. SRB is useful as a caries detector dye with subsequent inspection of histological material.
General description:	Sulforhodamine B (also known as SRB, kiton rhodamine B, lissamine rhodamine B, or sulphorhodamine B) is a bright-pink anionic, aminoxanthene dye carrying two sulfonate substituents.
Packaging:	5, 25 g in glass bottle

Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	room temp
Colour Index Number	45100
UNSPSC	12171500

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