Sphingomyelinase from Bacillus cereus
SIGMA/S9396 - buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)
Synonym: Sphingomyelin choline phosphohydrolase; Sphingomyelin phosphodiesterase
CAS Number: 9031-54-3
MDL Number: MFCD00132362
Product Type: Chemical
Stoichiometry: Sphingomyelin + H2O → N-Acylsphingosine + Choline phosphate
| foreign activity | Phospholipase C ≤1% |
| form | buffered aqueous glycerol solution |
| mol wt | 34.2 kDa |
| Quality Level | 200  |
| specific activity | ≥100 units/mg protein (Lowry) |
| storage temp. | 2-8°C |
| Application: | Sphingomyelinase from Bacillus cereus has been used: • in the study to analyse the effect of sphingomyelinase on Rubella virus infectivity in cell lines • in lipoprotein modification for the generation of cholesterol crystals in vitro • to aggregate LDL (low-density lipoprotein) |
| Application: | Sphingomyelinase has been used in a study to assess the interaction of actin with the HIV-1 accessory protein Nef. Sphingomyelinase has also been used in a study to investigate X-ray scattering as a quality-control tool for liposomal drug-delivery systems. |
| Biochem/physiol Actions: | Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin. |
| Biochem/physiol Actions: | Sphingomyelin phosphodiesterase (SMPD) participates in the metabolic reactions comprising sphingomyelin. SMPD has the ability to prevent colorectal carcinoma and colitis. |
| General description: | Sphingomyelinase (SMase) is a hydrolase, of 34.2 kDa and its active site contain as a minimum of two magnesium atoms. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C. |
| Physical form: | Solution in 50% glycerol containing 50 mM Tris-HCl, pH 7.5. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥100 units/mg protein (Lowry) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.4.12 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |