Superoxide dismutase–polyethylene glycol from bovine erythrocytes
SIGMA/S9549 - lyophilized powder, ≥1,350 units/mg protein
Synonym: PEG-SOD
MDL Number: MFCD00678115
Product Type: Chemical
| composition | Protein, ≥20% biuret |
| extent of labeling | ≥10 mol PEG per mol protein |
| form | lyophilized powder |
| mol wt | 5000 Da |
| Quality Level | 200  |
| 400 |
|
| specific activity | ≥1,350 units/mg protein |
| storage temp. | −20°C |
| Application: | Superoxide dismutase-polyethylene glycol from bovine erythrocytes has been used: • in the incubation of lung slices for measurement of dihydroethidium (DHE) fluorescence in cultured bovine pulmonary artery endothelial cells • as supplement in T cell culture to investigate its effect on cytokine production • in the preincubation of the carotid artery sections prior to DHE treatment |
| Biochem/physiol Actions: | Superoxide dismutase-polyethylene glycol (SOD-PEG) is effective than SOD in scavenging free radicals in injuries. It reduces oxidative damage in ovarian follicles cell culture and during ovarian tissue transplantation. SOD-PEG is also effective in scavenging superoxide in mitochondrial abnormalities. |
| General description: | Superoxide dismutase from bovine erythrocytes is a copper and zinc superoxide dismutase. Copper and zinc ions are coordinated by active site histidine residues. It has β-barrel structure. |
| Other Notes: | Superoxide dismutase from bovine erythrocytes coupled to methoxy-polyethylene glycol, M.W. 5,000, through secondary amine linkage. |
| Packaging: | Package size based on protein content |
| Physical form: | Lyophilized powder containing PEG plus 5% citrate buffer salts |
| Preparation Note: | Enzyme derived |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥1,350 units/mg protein |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |