Anti-SUMO-2 antibody produced in rabbit
SIGMA/S9571 - ~0.6 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-SMT3B; Anti-SMT3H2; Anti-Sentrin-2
MDL Number: MFCD03455941
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-SUMO-2 antibody produced in Rabbit (Cat. No. S9571) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing SUMO-2 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:1,000.
Immunofluorescence HeLa cells were fixed and permeabilized with methanol. Fixed cells were stained with 10 μg/mL Anti-SUMO-2 antibody produced in Rabbit (Cat. No. S9571) followed by 1:100 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887).
Immunoblotting Cell lysates were separated on SDS-PAGE and blotted with Anti-SUMO-2 (Cat. No. S9571). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:1,000. Lanes 1: HEK-293T cells transfected with human SUMO-2 2: Untransfected HEK-293T cells 3: HeLa nuclear cell lysate 4: HEK-293T cells transfected with human SUMO-2 + Inhibition with SUMO-2 immunizing peptide (human, 80-93) (10 μg/mL)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~0.6 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~15 kDa |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 10-20 μg/mL using HeLa cells |
| western blot: 0.5-1 μg/mL using nuclear extract of HeLa cells or cell extract of HEK293-T cells transfected with human SUMO-2 | |
| UniProt accession no. | P61956  |
| Application: | Anti-SUMO-2 antibody produced in rabbit has been used in immunoblotting and immunofluorescence. |
| Biochem/physiol Actions: | Anti-SUMO-2 recognizes human SUMO-2. |
| Biochem/physiol Actions: | Small ubiquitin-related modifier (SUMO-2) is essential for the cellular defense in hyperthermic cytotoxicity. Along with SUMO-3, SUMO2 is essential for cell survival post-heat shock. SUMO-2 has the ability to exist as self-modified polymers. SUMO-2 and SUMO-3 negatively regulate interferon induction. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Small ubiquitin-related modifier (SUMO-2) gene is mapped to human chromosome 17q25.1. Sumo-2 is related to SUMO-3 with a 86% sequence identity. It comprises a sumoylation motif at the residue lysine 11. |
| Immunogen: | synthetic peptide corresponding to amino acids 80-93 located at the C-terminal of human SUMO-2, conjugated to KLH. This sequence is identical in many species including rat, mouse, pig, and bovine SUMO-2 and is identical in human SUMO-3. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovien serum albumin and 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |