S-Gal®
SIGMA/S9811 - reagent for selection of recombinant bacterial clones
Synonym: 3,4-
CAS Number: 182805-65-8
Empirical Formula (Hill Notation): C19H22O9
Molecular Weight: 394.37
Linear Formula: C19H22O9
Product Type: Chemical
| assay | ≥95% (HPLC) |
| form | powder |
| grade | Molecular Biology |
| InChI | 1S/C19H22O9/c20-7-14-15(2 |
| InChI key | HDJJDRXZHJRVGA-DIKXUDHVSA |
| Quality Level | 100  |
| SMILES string | OC[C@H]1O[C@@H](Oc2cc3OC( |
| solubility | DMF: 50 mg/mL |
| sterility | non-sterile |
| storage temp. | room temp |
| suitability | suitable for β-galactosidase test |
| Application: | S-Gal is a patented autoclavable chromogenic substrate for β-galactosidase that is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype. |
| Application: | Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. S-Gal® is autoclavable and can be added to bacterial broth containing agar prior to autoclaving. |
| Biochem/physiol Actions: | When S-Gal® is cleaved by ß-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal® and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies. |
| Features and Benefits: | • More intense color contrast than X-gal • Excellent for use in automated colony counters • Autoclavable for easiest use • No need to make stock solutions |
| Features and Benefits: | When S-Gal is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies. S-Gal is autoclavable and can be added to your medium of choice prior to autoclaving. S-Gal is not light sensitive and does not require any protection from light sources. |
| General description: | S-Gal® is an autoclavable, chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. S-Gal® is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype. |
| Legal Information: | S-GAL is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Autoclavable, microwavable growth medium complete with S-Gal and IPTG. |
| Other Notes: | The ferric or Fe3+ ion is required for color development and must be added to any S-Gal® formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation. |
| Preparation Note: | Stock solutions of S-Gal® can be made by dissolving 50mg/ml in dimethyl formamide (DMF) and storing at -20C. Add S-Gal® (300 mg/L from stock solution ) and Ferric Ammonium Citrate (500mg/L) to agar media prior to autoclaving. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 - H335 |
| Precautionary statements | P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC) |
| Storage Temp. | room temp |
| UNSPSC | 12352200 |