Stem Cell Factor from mouse
SIGMA/S9915 - SCF, recombinant, expressed in E. coli, powder, suitable for cell culture
Synonym: SCF; c-Kit ligand
MDL Number: MFCD00132378
Product Type: Chemical
Bioassay EC50 is defined as the effective concentration of Stem Cell Factor from mouse that elicits a 50% attachment factor in cell based Bioassay.
SDS-PAGE The purity of recombinant Mouse SCF (Cat. No. S9915) was determined by SDS-PAGE of reduced SCF and staining with Coomassie Blue.
| assay | ≥95% (SDS-PAGE and HPLC) |
| biological source | mouse |
| color | white |
| form | powder |
| impurities | <1 EU/μg |
| mol wt | 18.3 kDa |
| packaging | pkg of 10 μg |
| pkg of 50 μg | |
| potency | ≤10 ng/mL ED50/EC50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| solubility | water: soluble |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P20826  |
| Analysis Note: | The biological activity is measured by the dose-dependent stimulation of the proliferation of human TF-1 cells. |
| Application: | SCF, also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors. |
| Application: | Stem Cell Factor from mouse has been used in the evaluation of in vitro 5-lipoxygenase inhibition by measuring the formation of LTC4. |
| Biochem/physiol Actions: | Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. |
| Biochem/physiol Actions: | Stem cell factor is responsible for cellular development, proliferation, migration and survival, specially observed with cardiac stem cells. This phenomenon is mediated by stem cell factor (SCF) through chemotaxis. SCF is involved in repair mechanism of myocardial infarctions. SCF signalling is responsible for the function and survival rate of interstitial cells of Cajal. Decreased level of SCF leads to gastrointestinal motility disorders. SCF might be associated with systemic vascular remodeling. SCF signalling is also believed to contribute to the pathogenesis of pulmonary hypertension. |
| General description: | Stem cell factor (SCF), also known as the c-kit ligand, is produced by the gastrointestinal smooth muscle cells, enteric neurons, endothelial cell, lung fibroblast and bone marrow adipocytes. The SCF gene is mapped to murine chromosome 10. Recombinant mouse stem cell factor (SCF) is a 18.3 kDa protein containing 164 amino acid residues. |
| Physical form: | Lyophilized from a 0.2 μm filtered buffered solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE and HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |