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Starch (HK) Assay Kit

SIGMA/SA20 - sufficient for 20 assays

Synonym: Starch Quantitation Kit

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-SA20-1KT 1 kit
 $238.00
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analyte chemical class(es) sugars
application(s) food and beverages
general analytical
Quality Level 100 
storage temp. 2-8°C
technique(s) photometry: suitable
usage sufficient for 20 assays
Application: This kit is for the quantitative, enzymatic determination of native starch in food and other materials using the amyloglucosidase-hexokinase-glucose 6-phosphate dehydrogenase enzymatic assay. The hydrolysis of starch to glucose is catalyzed by amyloglucosidase. Glucose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The increase in absorbance at 340 nm is directly proportional to the glucose concentration.
Storage Temp. 2-8°C
UNSPSC 12164500
Components glucose assay reagent 20 mL; starch assay reagent 1 mL/vial; starch assay standard 5 g

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