Anti-RS1 (66-78) antibody produced in rabbit
SIGMA/SAB1100315 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-RS; Anti-Retinoschisin 1; Anti-XLRS1
Product Type: Chemical
Immunofluorescence Anti-RS1 (66-78), Cat. No. SAB1100315: Immunofluorescence of HUVEC cells using Anti-RS1 (66-78), SAB1100315 (red), taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
Immunoblotting Lysate of HEK-293T cells overexpressing human RS1 was separated on SDS-PAGE, blotted with Anti-RS1 (66-78) (Cat. No. SAB1100315) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:500 2. Antibody dilution 1:500 + RS1 immunizing peptide (human, 66-78)
Immunofluorescence Graph Anti-RS1 (66-78), Cat. No. SAB1100315: Intensity analysis of Anti-RS1 (66-78) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different Antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~26 kDa |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:500-1:2,000 |
| UniProt accession no. | O15537   |
| Application: | Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if . |
| Biochem/physiol Actions: | Retinoschisin 1 (RS1) plays a role in binding of β-2-laminin, αβ-crystallin, phospholipid, galactose and Na/K ATPase−SARM1 (Sterile alpha and TIR motif-containing protein 1) complex. Mutations in the RS1 gene leads to a condition called as X-linked juvenile retinoschisis which leads to macular degradation in males and deterioration of vision. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Retinoschisin 1 (RS1) is a localized in the retina and pineal. Its is encoded by the X-linked retinoschisis (XLRS) gene and is 24 kDa. It consists of a signal sequence, RS1 domain, discoidin domain and a C-terminal domain. It is secreted by the retinal neurons as a homo-octameric complex. |
| Immunogen: | synthetic peptide corresponding to amino acids 66-78 of human RS1 |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |