Anti-SMOX (151-165) antibody produced in rabbit
SIGMA/SAB1101510 - IgG fraction of antiserum
Synonym: Anti-C20orf16; Anti-PAO; Anti-SMO; Anti-Spermine oxidase
Product Type: Chemical
Immunofluorescence Anti-SMOX (151-165) (RB): Cat. No. SAB1101510: Immunofluorescence of HUVEC cells using Anti-SMOX (151-165) (RB) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
Immunofluorescence Graph Anti-SMOX (151-165) (RB): Cat. No. SAB1101510: Intensity analysis of Anti-SMOX (151-165) (RB) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6400) as compared with the negative control.
Immunoblotting Lysate of HEK-293T cells overexpressing human SMOX was separated on SDS-PAGE, blotted with Anti-SMOX (151-165) (Cat. No. SAB1101510) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:500 2. Antibody dilution 1:500 + SMOX immunizing peptide (human,151-165)
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | ~56 kDa |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:500-1:2,000 |
| UniProt accession no. | Q9NWM0   |
| Application: | Anti-SMOX (151-165) antibody produced in rabbit has been used in western blotting. |
| Biochem/physiol Actions: | Spermine oxidase (SMOX) catabolizes spermine to spermidine, 3-aminopropanal and hydrogen peroxide. This catalytic activity of SMOX leads to apoptosis and DNA damage in gastric epithelial cells. It is highly expressed in H. pylori infected gastritis tissues and in ulcerative colitis (UC). Induced SMOX expression in breast cancer using spermine analogs is regarded as an effective anticancer treatment regime. It is regarded as a key marker correlating chronic inflammation with epithelial carcinogenesis. Elevation in the SMO levels is observed in inflammatory bowel disease and is implicated in the immunopathogenesis of Citrobacter rodentium infection mediated colitis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Spermine oxidase (SMOX) gene mapped to human chromosome 20p13, is a flavin adenine dinucleotide (FAD)-dependent oxidase. It encodes nucleus and cytoplasmic specific splice variants. |
| Immunogen: | synthetic peptide corresponding to amino acids 151-165 of human SMOX |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |