Anti-JMJD3 (N-term) antibody produced in rabbit
SIGMA/SAB1300005 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-JMJD3; Anti-JmjC domain-containing protein 3; Anti-Jumonji domain-containing protein 3; Anti-KIAA0346
Product Type: Chemical
Immunohistochemistry Anti-JMJD3 (N-term) Antibody: Cat. No. SAB1300005: JMJD3 in human brain tissue with Anti-JMJD3 (N-term) Antibody at 40 μg/mL.
Immunoblotting Anti-JMJD3 (N-term) Antibody: Cat. No. SAB1300005: JMJD3 in HeLa cell line lysates with Anti-JMJD3 (N-term) Antibody at 4.0 μg/mL.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_001073893   |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:1000 |
| western blot: 1:100-1:500 | |
| UniProt accession no. | O15054 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed. |
| Immunogen: | JMJD3 (NP_001073893, 1-40) This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human JMJD3. |
| Physical form: | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |