Anti-PI4KCA (N-term) antibody produced in rabbit
SIGMA/SAB1300025 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-PI4K230; Anti-
Product Type: Chemical
Immunohistochemistry Anti-PI4KCA (N-term) Antibody: Cat. No. SAB1300025: PI4KCA in human breast carcinoma tissue with Anti-PI4KCA (N-term) Antibody at 40 μg/mL.
Immunoblotting Anti-PI4KCA (N-term) Antibody: Cat. No. SAB1300025: PI4KCA in human placenta tissue lysates with Anti-PI4KCA (N-term) Antibody at 4.0 μg/mL.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_002641   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:50-1:100 |
| indirect ELISA: 1:1000 | |
| western blot: 1:100-1:500 | |
| UniProt accession no. | P42356 |
| General description: | Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. This gene encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. Two transcript variants encoding different isoforms have been described for this gene. Variant 1 is alternatively spliced at the 5′ end compared to transcript variant 2. However, it maintains the same reading frame and encodes an isoform 1 (97 kDa) which is truncated at the N-terminus compared to isoform 2 (230 kDa). Isoform 1 has enzymatic properties characteristic of type II PI 4-kinases. Variant 2 is full-length, and encodes the longer isoform 2 with a different N-terminus compared to isoform 1. Isoform 2 has enzymatic properties characteristic of type III PI 4-kinases. |
| Immunogen: | PI4KCA (11-47) This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PI4KCA. |
| Physical form: | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |