Anti-UBA2 (N-term) antibody produced in rabbit
SIGMA/SAB1300146 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-HRIHFB2115; Anti-SUMO-1 activating enzyme subunit 2; Anti-Ubiquitin-like 2 activating enzyme E1B; Anthracycline-
Product Type: Chemical
Immunohistochemistry Anti-UBA2 (N-term) Antibody: Cat. No. SAB1300146: UBA2 in human breast carcinoma tissue with Anti-UBA2 (N-term) Antibody at 40 μg/mL.
Immunohistochemistry Anti-UBA2 (N-term) Antibody: Cat. No. SAB1300146: UBA2 in human breast carcinoma tissue with Anti-UBA2 (N-term) Antibody at 40 μg/mL.
Immunoblotting Anti-UBA2 (N-term) Antibody: Cat. No. SAB1300146: UBA2 in mouse brain tissue lysates with Anti-UBA2 (N-term) Antibody at 4.0 μg/mL.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_005490   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:50-1:100 |
| indirect ELISA: 1:1000 | |
| western blot: 1:100-1:500 | |
| UniProt accession no. | Q9UBT2 |
| General description: | Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43% sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8. |
| Immunogen: | UBA2 (Q9UBT2, 1-30) This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human UBA2. |
| Physical form: | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |