Anti-SUMO4 (M55 Wild type) antibody produced in rabbit
SIGMA/SAB1300170 - saturated ammonium sulfate (SAS) precipitated, buffered aqueous solution
Synonym: Anti-SMT3 suppressor of mif two 3 homolog 2; Anti-SMT3H4; Anti-SUMO-4; Anti-Small ubiquitin-like modifier 4; Anti-Small ubiquitin-like protein 4
Product Type: Chemical
Immunohistochemistry Anti-SUMO4 (M55 Wild Type) Antibody: Cat. No. SAB1300170: SUMO4 in human breast carcinoma tissue with Anti-SUMO4 (M55 Wild Type) Antibody at 40 μg/mL.
Immunohistochemistry Anti-SUMO4 (M55 Wild Type) Antibody: Cat. No. SAB1300170: SUMO4 in human hepatocarcinoma tissue with Anti-SUMO4 (M55 Wild Type) Antibody at 40 μg/mL.
Immunoblotting Anti-SUMO4 (M55 Wild Type) Antibody: Cat. No. SAB1300170: SUMO4 in HepG2 cell line lysates with Anti-SUMO4 (M55 Wild Type) Antibody at 4.0 μg/mL.
| antibody form | saturated ammonium sulfate (SAS) precipitated |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_001002255   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:50-1:100 |
| indirect ELISA: 1:1000 | |
| western blot: 1:100-1:500 | |
| UniProt accession no. | Q6EEV6 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | SUMO4 is a member of the SUMO gene family. This family of small ubiquitin-related modifiers covalently modify target lysines in proteins and control the target proteins′ subcellular localization, stability, or activity. Upon oxidative stress, SUMO4 conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. This protein may also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity. Covalent attachment to SUMO4 substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I. In contrast to SUMO1, SUMO2 and SUMO3, SUMO4 seems to be insensitive to sentrin-specific proteases due to the presence of Pro-90. This may impair processing to mature form and conjugation to substrates. SUMO4 is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-kappa-B-dependent transcription of the IL12B gene. The M55V substitution has been associated with type I diabetes. This antibody is generated from rabbits immunized with the KLH conjugated peptide CEPRGLS(M)KQIRFRFG selected from human SUMO4. This antibody is peptide affinity purified using peptides CEPRGLS(M)KQIRFRFG (positive selection) and CEPRGLS(V)KQIRFRFG (negative selection). |
| Immunogen: | SUMO4 (NP_001002255, ) This antibody is generated from rabbits immunized with the KLH conjugated peptide CEPRGLS(M)KQIRFRFG selected from human SUMO4. This antibody is peptide affinity purified using peptides CEPRGLS(M)KQIRFRFG (positive selection) and CEPRGLS(V)KQIRFRFG (negative selection). |
| Physical form: | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |