Anti-DKK2 (C-term) antibody produced in rabbit
SIGMA/SAB1300210 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-Dickkopf related protein-2
Product Type: Chemical
Immunohistochemistry Anti-DKK2 (C-term) Antibody: Cat. No. SAB1300210: DKK2 in human lung carcinoma tissue with Anti-DKK2 (C-term) Antibody at 40 μg/mL.
Immunohistochemistry Anti-DKK2 (C-term) Antibody: Cat. No. SAB1300210: DKK2 in human breast carcinoma tissue with Anti-DKK2 (C-term) Antibody at 40 μg/mL.
Immunoblotting Anti-DKK2 (C-term) Antibody: Cat. No. SAB1300210: DKK2 in A375 cell line lysates with Anti-DKK2 (C-term) Antibody at 4.0 μg/mL.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_055236   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:50-1:100 |
| indirect ELISA: 1:1000 | |
| western blot: 1:100-1:500 | |
| UniProt accession no. | Q9UBU2 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The 259-amino acid DKK2 protein, like DKK1, DKK3, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cys residues each. The second cys region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed expression of 4.0- and 4.5-kb DKK2 transcripts in heart, brain, skeletal muscle, and lung. Western blot analysis showed that DKK2 is secreted as a 15- to 17-kD protein. Functional analysis determined that DKK2 does not block Xenopus Wnt8 induction of a secondary axis in frog embryos. This polyclonal antibody was raised against a peptide corresponding to a sequence selected for the C-terminal region of human DKK2. |
| Immunogen: | DKK2 (Q9UBU2, 229-261) This polyclonal antibody was raised against a peptide corresponding to a sequence selected for the C-terminal region of human DKK2. |
| Physical form: | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |