Anti-PDX1 (N-term) antibody produced in rabbit
SIGMA/SAB1300633 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-E3-binding protein; Anti-Pyruvate dehydrogenase protein X component; Dihydrolipoamide dehydrogenase-
Product Type: Chemical
Immunohistochemistry Anti-PDX1 (N-term) Antibody: Cat. No. SAB1300633: PDX1 in human breast carcinoma tissue with Anti-PDX1 (N-term) Antibody at 40 μg/mL.
Immunoblotting Anti-PDX1 (N-term) Antibody: Cat. No. SAB1300633: PDX1 in HEK 293 cell line lysates either non-transfected (Lane 1) or transiently transfected (Lane 2) with Anti-PDX1 (N-term) Antibody at 4.0 μg/mL.
Immunoblotting Anti-PDX1 (N-term) Antibody: Cat. No. SAB1300633: PDX1 in mouse heart tissue lysates with Anti-PDX1 (N-term) Antibody at 4.0 μg/mL.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_000200   |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:50-1:100 |
| indirect ELISA: 1:1000 | |
| UniProt accession no. | P52945 |
| General description: | PDX1, located in the mitochondrial matrix, is required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by noncovalent bonds. Defects in PDHX are a cause of lacticacidemia. PDX1 belongs to the 2-oxoacid dehydrogenase family and contains 1 lipoyl-binding domain. |
| Immunogen: | PDX1 (9-40) This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PDX1. |
| Physical form: | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |