ANTI-MLCK(N-TERMINAL) antibody produced in rabbit
SIGMA/SAB1305415 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Myosin light chain kinase
Product Type: Chemical
Immunoblotting Anti-MLCK (N-term): Cat. No. SAB1305415: Western blot analysis of Anti-MLCK-long Pab in Mouse brain tissue lysate. MLCK-long (Arrow) was detected using purified Pab. Secondary HRP-Anti-Rabbit was used for signal visualization with chemiluminescence.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| form | buffered aqueous solution |
| mol wt | 210715 Da |
| NCBI accession no. | NP_444253.3   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:50-1:100 |
| western blot: 1:1000 | |
| UniProt accession no. | Q15746 |
| Biochem/physiol Actions: | Myosin light chain kinase (MLCK) is activated by the binding of Ca2+/calmodulin. It has a role in smooth muscle contraction. The kinase phosphorylates the regulatory light chain of smooth muscle myosin. This stimulates ATPase activity of the myosin heads and leads to the myosin power stroke, which is crucial for muscle contraction. MLCK also assists the interaction of myosin with actin. Loss of function of the protein has been linked to megacystis microcolon intestinal hypoperistalsis syndrome. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Myosin light chain kinase (MLCK) is a serine/threonine kinase and the gene encoding it is localized on human chromosome 3q21.1. |
| Physical form: | Supplied in PBS with 0.09% (W/V) sodium azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |