MONOCLONAL ANTI-BETA-ACTIN antibody produced in mouse
SIGMA/SAB1305554 - clone 137CT26.1.1, IgG fraction of antiserum, buffered aqueous solution
Synonym: ACTB; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; Beta-actin
Product Type: Chemical
Immunoblotting Monoclonal Anti-Beta-Actin: Cat. No. SAB1305554: Western blot analysis of Anti-ACTB Antibody in K562, HL-60,HeLa cell line, Mouse spleen, Mouse liver tissue lysates, Mouse NIH-3T3 cell line lysate and Mouse cerebellum, Mouse brain tissue lysates (35 μg/lane).
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 137CT26.1.1, monoclonal |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | 41737 Da |
| NCBI accession no. | NP_001092.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 1:10-1:50 |
| immunohistochemistry: 1:25 | |
| western blot: 1:1000 | |
| UniProt accession no. | P60709 |
| Application: | Monoclonal Anti-Beta-Actin antibody produced in mouse has been used in western blotting at a dilution of 1:1000. |
| Biochem/physiol Actions: | β-actin (ACTB) forms the core of the cell cytoskeleton. The polymerization of β-actin is essential for cell physiology. It may serve as a potential chemotherapeutic agent. Mutation in the ACTB gene is implicated in sensory hearing loss, delayed-onset generalized dystonia and a combination of ventral midline malformations. A loss-of-function mutation in the ACTB gene may cause intellectual disability. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | β-actin (ACTB), a member of the actin multigene family, is an isoform of actin-associated with non-muscle cells. ACTB is a housekeeping gene mapped to human chromosome 7p22.1. |
| Physical form: | Supplied in PBS with 0.09% (W/V) sodium azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |