ANTI-HIS TAG antibody produced in rabbit
SIGMA/SAB1306082 - purified immunoglobulin, buffered aqueous solution
Synonym: Rabbit anti-hexa histidine tag
Product Type: Chemical
Immunoblotting Anti-His Tag : Cat. No. SAB1306082: Western blot analysis of His Tag Antibody in his-tag TBB1 protein (35 μg/lane).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| form | buffered aqueous solution |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:1000 |
| UniProt accession no. |  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Histidine has a strong association with immobilized metal ion matrices. This is because the imidazole ring has electron donor groups that form coordination bonds with the transition metal that is immobilized. Hexahistidine tag (His-tag) consists of four to six histidines that are fused genetically. It is mainly used to purify proteins by immobilized metal-ion affinity chromatography (IMAC). His-tags are added at the amino or carboxy termini of proteins. |
| Physical form: | Supplied in PBS with 0.09% (W/V) sodium azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |