ANTI-LC3 (APG8B) (N-TERM) antibody produced in rabbit
SIGMA/SAB1306611 - purified immunoglobulin, buffered aqueous solution
Synonym: Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1ALC3; MAP1LC3B; Microtubule-
Product Type: Chemical
Immunofluorescence Anti-LC3 (APG8B) (N-term): Cat. No. SAB1306611: Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B)(N-term) . U251 cells(right) were treated with Chloroquine (50 ?M,16h). LC3 antibody was diluted at 1:25 dilution.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| form | buffered aqueous solution |
| mol wt | 14688 Da |
| NCBI accession no. | NP_073729.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 1:100 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:1000 | |
| UniProt accession no. | Q9GZQ8 |
| Biochem/physiol Actions: | MAP1LC3B-I (microtubule associated protein 1 light chain 3 beta-I) conjugates with MAP1LC3B-II before being inserted into both inner and outer membranes of the growing autophagic vesicle during autophagy. The cellular distribution and processing of MAP1LC3B facilitates monitoring of autophagy. Cells that lack MAP1LC3B are found to be defective in the formation of autophagic vesicles, with altered phenotypes associated with the role of autophagy in energy homeostasis and removal of protein aggregates. It can serve as an autophagic marker. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The gene LC3B or MAP1LC3B (microtubule associated protein 1 light chain 3 beta) is a homolog of the ATG8 gene, an autophagy-related gene in yeast. The encoded protein is called pro-MAP1LC3B, a pro-form that is cleaved by ATG4 to produce a cytosolic form called MAP1LC3B-I. |
| Physical form: | Supplied in PBS with 0.09% (W/V) sodium azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |