Monoclonal Anti-AKT1 antibody produced in mouse
SIGMA/SAB1400008 - clone 4B9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-MGC99656; Anti-PKB; Anti-PRKBA; Anti-RAC; Anti-RACALPHA
MDL Number: MFCD03096597
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to AKT1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 μg/mL]
Western Blotting AKT1 monoclonal antibody, clone 4B9 Western Blot analysis of AKT1 expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (78.54 kDa).
ELISA Detection limit for recombinant GST tagged AKT1 is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4B9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P31749  |
| General description: | The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. (provided by RefSeq) |
| Immunogen: | AKT1 (AAH00479.1, 1 a.a. ~ 480 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSDVAIVKEGWLHKRGEYIKTWRPR |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |