Monoclonal Anti-RHOA antibody produced in mouse
SIGMA/SAB1400018 - clone 1C1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ARH12; Anti-ARHA; Anti-RHO12; Anti-RHOH12
MDL Number: MFCD09265373
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to RHOA on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between MAPK8 and RHOA. HeLa cells were stained with anti-MAPK8 rabbit purified polyclonal 1:1200 and anti-RHOA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting RHOA monoclonal antibody, clone 1C1 Western Blot analysis of RHOA expression in HeLa.
Western Blotting Western Blot analysis of RHOA expression in transfected 293T cell line by RHOA monoclonal antibody, clone 1C1. Lanes Lane 1: RHOA transfected lysate (21.8 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (46.97 kDa).
ELISA Detection limit for recombinant GST tagged RHOA is 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1C1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1λ |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P61586  |
| Immunogen: | RHOA (AAH01360, 1 a.a. ~ 193 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAAIRKKLVIVGDGACGKTCLLIVF |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |