Monoclonal Anti-BUB1B antibody produced in mouse
SIGMA/SAB1400031 - clone 3F2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-BUB1beta; Anti-BUBR1; Anti-Bub1A; Anti-MAD3L; Anti-SSK1; Anti-hBUBR1
MDL Number: MFCD04040371
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to BUB1B on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1 μg/mL]
Western Blotting BUB1B monoclonal antibody, clone 3F2 Western Blot analysis of BUB1B expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (39.93 kDa).
ELISA Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3F2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgGκ |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O60566  |
| General description: | This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. (provided by RefSeq) |
| Immunogen: | BUB1B (AAH18739, 1 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAAVKKEGGALSEAMSLEGDEWELS |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |