Monoclonal Anti-CKMT1B antibody produced in mouse
SIGMA/SAB1400052 - clone 2C8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CKMT; Anti-CKMT1; Anti-UMTCK
Product Type: Chemical
Western Blotting Western Blot analysis of CKMT1B expression in transfected 293T cell line by CKMT1B monoclonal antibody, clone 2C8. Lanes Lane 1: CKMT1B transfected lysate (47 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of CKMT1B over-expressed 293 cell line, cotransfected with CKMT1B Validated Chimera RNAi. Blot probed with CKMT1B monoclonal antibody, clone 2C8. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting CKMT1B monoclonal antibody, clone 2C8. Western Blot analysis of CKMT1B expression in A-431.
Western Blotting QC Western Blot detection against Immunogen (35.75 kDa).
ELISA Detection limit for recombinant GST tagged CKMT1B is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2C8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL |
| General description: | Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. (provided by RefSeq) |
| Immunogen: | CKMT1B (NP_066270, 327 a.a. ~ 417 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence GVHIKLPLLSKDSRFPKILENLRLQ |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |