Anti-CYP3A4 antibody produced in mouse
SIGMA/SAB1400064 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-CP33; Anti-CP34; Anti-CYP3A; Anti-CYP3A3; Anti-HLP; Anti-MGC126680; Anti-NF-25; Anti-P450C3; Anti-P450PCN1
MDL Number: MFCD01633033
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of antibody to CYP3A4 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 μg/mL]
Western Blotting Western Blot analysis of CYP3A4 expression in transfected 293T cell line by CYP3A4 polyclonal antibody. Lanes Lane 1: CYP3A4 transfected lysate (55.33 kDa). Lane 2: Non-transfected lysate.
Western Blotting CYP3A4 polyclonal antibody. Western Blot analysis of CYP3A4 expression in HepG2.
Western Blotting CYP3A4 polyclonal antibody. Western Blot analysis of CYP3A4 expression in human liver.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | P08684  |
| Application: | Anti-CYP3A4 antibody produced in mouse has been used in the immunohistochemical analysis (1:100) and immunofluorescence staining (1:200). |
| Biochem/physiol Actions: | Cytochrome P450 3A4 (CYP3A4) plays a key role in the metabolism of several antineoplastic drugs. It aids in the catalysis of a wide range of substrates. CYP3A4 can detoxify bile acids. CYP3A4 gene mutations can disrupt the metabolism of commonly used medications, resulting in harmful blood concentrations of drugs. Such CYP3A4 polymorphisms may also affect the detoxification of bile acid. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cytochrome P450 3A4 (CYP3A4), the ubiquitous cytochrome P450 (CYP) seen in the adult liver and intestine, belongs to the CYP3A subfamily. The CYP3A4 gene with 13 exons is located on human chromosome 7q22.1. |
| Immunogen: | CYP3A4 (AAI01632.1, 1 a.a. ~ 503 a.a) full-length human protein. Sequence MALIPDLAMETWLLLAVSLVLLYLY |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |