Anti-DDX11 antibody produced in mouse
SIGMA/SAB1400075 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-CHL1; Anti-CHLR1; Anti-KRG2; Anti-MGC133249; Anti-MGC9335
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to DDX11 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of DDX11 expression in transfected 293T cell line by DDX11 polyclonal antibody. Lanes Lane 1: DDX11 transfected lysate (106.7 kDa). Lane 2: Non-transfected lysate.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | Q96FC9  |
| Application: | Anti-DDX11 antibody produced in mouse has been used in western blotting. |
| Biochem/physiol Actions: | DEAD/H-box helicase 11 (DDX11) maintains the cohesion of chromosome arms and centromeres. It plays an important role in genomic stability. Downregulation of DDX11 in melanoma cells causes inhibition of melanoma cell proliferation, defective chromosome segregation and apoptosis. DDX11 is required for the formation of heterochromatin. Mutations in this gene is linked to Warsaw breakage syndrome (WABS). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | DEAD/H-box helicase 11 (DDX11) gene is located on human chromosome 12p11.21. DDX11 encodes an iron sulphur containing DNA helicase. DDX11 is overexpressed in melanomas. |
| Immunogen: | DDX11 (AAH50522.1, 1 a.a. ~ 970 a.a) full-length human protein. Sequence MANETQKVGAIHFPFPFTPYSIQED |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |