Anti-E4F1 antibody produced in mouse
SIGMA/SAB1400082 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-E4F; Anti-MGC99614
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to E4F1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of E4F1 expression in transfected 293T cell line by E4F1 polyclonal antibody. Lanes Lane 1: E4F1 transfected lysate (86.24 kDa). Lane 2: Non-transfected lysate.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | Q66K89  |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence. (provided by RefSeq) |
| Immunogen: | E4F1 (AAH80524.1, 1 a.a. ~ 784 a.a) full-length human protein. Sequence MEGAMAVRVTAAHTAEAQAEAGREA |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |