Monoclonal Anti-EEF1D antibody produced in mouse
SIGMA/SAB1400087 - clone 4B12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-EF-1D; Anti-FLJ20897; Anti-FP1047
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to EEF1D on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting EEF1D monoclonal antibody, clone 4B12. Western Blot analysis of EEF1D expression in human pancreas.
Western Blotting QC Western Blot detection against Immunogen (35.75 kDa).
ELISA Detection limit for recombinant GST tagged EEF1D is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4B12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P29692  |
| General description: | This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | EEF1D (NP_115754, 1 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MRSGKASCTLETVWEDKHKYEEAER |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |