Monoclonal Anti-HDAC1 antibody produced in mouse
SIGMA/SAB1400121 - clone 5C11, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DKFZp686H12203; Anti-GON10; Anti-HD1; Anti-RPD3; Anti-RPD3L1
MDL Number: MFCD01633420
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between NFKB1 and HDAC1. HeLa cells were stained with anti-NFKB1 rabbit purified polyclonal 1:1200 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting Western Blot analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody, clone 5C11. Lanes Lane 1: HDAC1 transfected lysate (55.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting HDAC1 monoclonal antibody, clone 5C11 Western Blot analysis of HDAC1 expression in HeLa S3 NE.
Western Blotting HDAC1 monoclonal antibody, clone 5C11. Western Blot analysis of HDAC1 expression in Raw 264.7.
Western Blotting HDAC1 monoclonal antibody, clone 5C11. Western Blot analysis of HDAC1 expression in NIH/3T3.
Western Blotting HDAC1 monoclonal antibody, clone 5C11. Western Blot analysis of HDAC1 expression in PC-12.
Western Blotting QC Western Blot detection against Immunogen (78.76 kDa).
ELISA Detection limit for recombinant GST tagged HDAC1 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5C11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q13547  |
| Biochem/physiol Actions: | Histone deacetylase 1 (HDAC1) is an epigenetic factor. It is part of the innate antiviral response. The protein has roles in DNA repair, splicing, regulation of gene expression and cell division. It deacetylates lysine residues of histone H3 and H4. HDAC1 has been shown to have a role in various cancers. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Histone deacetylase 1 (HDAC1) is mainly expressed in the nucleus. It belongs to class I of histone deacetylases and the gene encoding it is localized on human chromosome 1p35.2. |
| Immunogen: | HDAC1 (AAH00301, 1 a.a. ~ 482 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAQTQGTRRKVCYYYDGDVGNYYYG |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |